Coding

Part:BBa_K4160002:Design

Designed by: Femi Hesen, Wouter Langers, Floor van Boxtel   Group: iGEM22_TU-Eindhoven   (2022-10-09)


IL-6RB


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 531
    Illegal PstI site found at 692
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 531
    Illegal PstI site found at 692
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 9
    Illegal BglII site found at 195
    Illegal BglII site found at 459
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 531
    Illegal PstI site found at 692
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 531
    Illegal PstI site found at 692
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Mutation of GAC to GAT (bp 12) to make it RCF[1000] compatible


Source

Expression vector pLeo619. Sequence was found through Genbank https://www.ncbi.nlm.nih.gov/nuccore/MG437012

References